Purification and some properties of fungal Xylanase

Partial purification of xylanase produced by Aspergillus niger strain AUMC 14230 is carried out with 80% saturation ammonium sulfate under pH5.0 as first step. The second step in partial Acetone 2.5/1 (v/v). last gill filtrations which give the highest specific from both enzymes.  Many factors affecting on purified enzymes. best activity was till 72 hrs and decreased (8.8% & 10.9%), respectively after hrs. Xylan (1mg) concentration substrate to produce relative 100% for enzyme.  We found that glucose has an inhibitory effect enzymes activity. Optimum temperature 50oC, increased productivity addition Ca2+, Ba2+, Mg2+ EDTA strongly enhanced activity, While, Co2+ Ag+ productivity. On other hand, Fe2+, Cu2+ Mn2+ showed moderate inhibition.